What are the features of other E. coli IMPACT vectors?
Two additional pTYB C-terminal fusion vectors, pTYB3 (NEB #N6703) and pTYB4 (NEB #N6704), are available for cloning a target gene in which the C-terminus of the target protein is fused to the intein-CBD tag. pTYB3 and pTYB4 contain an NcoI site, overlapping the initiating methionine codon, in place of the NdeI site in pTYB1 and pTYB2, respectively. Digestion of the insert with BspHI, BspLU11I and AflIII can also generate NcoI-compatible overhangs. pTXB1 (NEB #N6707) and pTXB3 (NEB #N6708) are IMPACT C-terminal fusion vectors using an engineered mini-intein (198 residues) from the gyrAgene of Mycobacterium xenopi. These vectors are designed for fusing the C-terminus of a target protein to the Mxe intein-CBD tag for protein purification and generation of C-terminal thioester tagged proteins for labeling and protein ligation [See Appendix IV in IMPACT-CN manual and Appendix II in TWIN manual; Evans, T.C., Benner, J., and Xu, M.-Q.(1998) Semisynthesis of cytotoxic proteins using a modified
