What are the factors that may affect the result of the Gram-staining method?
A. The main factor that influences the result of a Gram stain (whether an organism is classified as Gram positive or Gram negative) is the structure of their cell wall. The Gram positive cell wall is much thicker than that of the gram negative organism, thus it retains the dark purple stain. The Gram negative cell wall is much thinner, and it is readily decolorized with alchohol.
