What are the different starting materials that I can use for Real-Time PCR?
The starting materials for Real-Time PCR can be RNA, genomic DNA, or plasmid DNA. RNA must be reverse-transcribed into complementary DNA (cDNA) before PCR. More recently, kits have become available that can perform RT-qPCR (RT = Reverse Transcription) directly from cells without the need for a separate RNA synthesis step.
