What are the different normalization options in GeneSpring?
A.There are 16 different normalization options in GeneSpring including intensity dependent (Lowess) normalization, global per chip or per gene normalizations, data transformations options, normalization to positive or negative controls, normalization to specified samples and more. These may be used in any combination, although generally if you have references for each gene you will not want to employ any of the other options. Similarly, if you are normalizing to positive controls you generally will not want to employ the per experiment normalization option.
