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What are the differences among dead volume, dwell volume, void volume, extra-column volume, etc.?

April 26, 2017dead differences dwell void volume

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What are the differences among dead volume, dwell volume, void volume, extra-column volume, etc.?

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The different “volumes” in an HPLC system are confusingly named and in some cases overlap. Briefly: • dead volume is the volume of liquid inside the column (also called the void volume) • dwell volume is the volume of liquid in a gradient system between the point at which the gradient is formed and the head of the column • void volume is the volume of liquid inside the column • extra-column volume is the volume of liquid that contributes to band broadening in the system, exlcuding the dead volume of the column.