What are the current PCR methods?
• Used for Toxoplasma, Ehrlichia, Bartonella, FeLV, Mycobacterium, Feline Herpes, Equine Herpes. • Difficulty interpreting results because PCR indicates organisms present but not causing disease. For example, Toxoplasma PCR on cats with uveitis: 19% PCR Positive compared with cats without uveitis 9% positive 2) Nested PCR Method: • Used to detect target organisms at low concentrations • Utilizes a second round of amplification • Main disadvantage is turnover time–requires two runs 3) Reverse Transcription PCR (RT-PCR) Method • Used for FIP, Canine Distemper • Amplifies an RNA template (i.e.
