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What are the critical parameters for successful ELISPOT assay?

April 26, 2017assay critical elispot parameters successful

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What are the critical parameters for successful ELISPOT assay?

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It is critically important to use a high affinity binding PVDF membrane plate, rather than regular ELISA plate or nitrocellulose membrane plate. Additionally, highest affinity antibodies for capture work best in this assay. Empirical optimization of conditions such as cell density, stimulus/mitogen, and kinetics is necessary.