What are the characteristics of OSNA?
The method is characterised by • No requirement for an RNA purification step. • A short reaction time consistent with the intra-operative use of the system. • The use of 6 primers that lead to a high specificity for the target mRNA. • Optimal Assay Design avoiding the amplification of CK19 Pseudogenes. • Gene-amplification at a constant temperature of 65°C to avoid genomic DNA amplification.
