What are the advantages to using nylon versus nitrocellulose membranes in nucleic acid applications?
A. Nylon membranes are less brittle and easier to handle than nitrocellulose, making them ideal for reprobing. They also respond more robustly to various environmental storage conditions than nitrocellulose. Nylon’s highly hydrophilic nature makes prewetting unnecessary, and nylon membranes have much higher binding capacities than nitrocellulose for nucleic acids. For more information on the use of Nylon membranes for Southerns and other nucleic acid applications please click here.
