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What are the advantages of using nylon for performing Southern and Northern blots?

April 26, 2017advantages blots northern nylon Performing southern

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What are the advantages of using nylon for performing Southern and Northern blots?

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DNA and RNA can be permanently bound to nylon, as opposed to nitrocellulose where the DNA may wash off during hybridization and post-hybridization washes. Nylon is also more durable than nitrocellulose, which makes it well suited for numerous rounds of stripping and reprobing.