What are the advantages of the wheat germ expression system compared with other systems for synthesizing recombinant proteins?
High quality protein synthesis in a wide temperature range: Compared with those produced in conventional cell lysates, the proteins synthesized in the wheat germ cell-free expression system are more likely to be properly folded and soluble. Furthermore, they are much less prone to degrade, because the system contains only negligible amounts of endogenous proteases. In addition, the system permits translation in a wide temperature range. The optimum translation temperature lies between 15 ºC and 26 ºC. In this temperature range, protein yield is nearly constant. Translation can also be carried out at temperatures lower than 15 ºC. This makes it possible to synthesize proteases, which are self-destructive at room temperature, and proteins that lack high-temperature stability. No necessity for codon optimization: The system shows little codon usage preference. As a result, even proteins encoded by AT-rich cDNAs, e.g., malaria proteins, can be synthesized without codon optimization. Fewer
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