What are the advantages of the NCode™ qRT-PCR system?
The NCode™ First strand cDNA synthesis system allows for generation of a “universal” cDNA that is generated from all RNA species present in the input samples. This is what allows housekeeping genes or alternate normalizers to be used for amplification within the same sample. This cDNA can be archived, or stored for use at a later time for analysis of other small RNAs. This can be essential when samples are precious and it is not a possibility with miRNA specific qRT-PCR assays. Since standard custom oligos are used for the qPCR step, this system is generally less costly than alternate methods. We also provide general design guidelines for primers, which allows for design and detection of novel miRNAs or other small RNAs for which there is no validated miRNA specific assay available.
