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What are the advantages of synthetic libraries over conventional protocols for creation of diversity?

April 26, 2017advantages conventional creation Diversity Libraries protocols synthetic

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What are the advantages of synthetic libraries over conventional protocols for creation of diversity?

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Conventional protocols for library creation incorporate a large fraction of unwanted randomness (e.g. error-prone PCR) and strict limitations in disjointing of adjacent SNPs (e.g. DNA shuffling). Synthetic degenerated libraries limit the introduction of mutations only to those sites and with the exact frequencies that are determined by the scientist. In addition, adjacent SNPs will be shuffled independently of their proximity. It is the method of choice to create rational diversity.