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What are the advantages of selection with antibiotics over fluorescent sorting using GFP?

April 26, 2017advantages antibiotics fluorescent gfp selection Sorting

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What are the advantages of selection with antibiotics over fluorescent sorting using GFP?

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Selection of transduced cells can be accomplished using either puromycin or FACS selecting for tGFP expressers. That being said, either option is effective. However, for completely pure, transduced cell populations, selection with puromycin is suggested.