What are the advantages of phage display screening over other methods of library screening?
The main advantage of phage display over other technologies is the ease with which one can screen large numbers of clones. Standard screening of cDNA libraries, such as those expressed in phage lambda, is limited by the number of plaques or colonies which can be screened by hybridization, typically on the order of 104. Synthetic random peptide libraries are typically screened on grids of pins, where binding sequences are identified by position, or on beads in suspension, where bound sequences are identified by sequencing a tag affixed to the selected bead. These technologies also limit the maximum number of random peptides that can be screened to 103-104 different sequences. If synthetic peptides are screened in solution, libraries can contain as many as 1015 different sequences, but the requirement for sufficient material to sequence (~1 g peptide) requires such low stringency during binding that enrichments of only 100 to 1000-fold are possible, resulting in selection of an enormous
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