What are the advantages of M13 over other phage used for phage display applications?
M13 and the closely related filamentous bacteriophages fd and f1 are non-lytic, meaning that they do not lyse the host during phage production. This greatly simplifies the intermediate phage purification steps between rounds of panning, as a simple PEG precipitation step is sufficient to separate the phage from almost all contaminating cellular proteins. In contrast, other phage that have been used for phage display (T7, T4, lambda) are all lytic, necessitating additional time-consuming purification steps between rounds to avoid panning amplified phage in the presence of cellular proteins (including proteases which can degrade your target during panning).
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