What are the advantages of hollow fiber cell culture for endothelial cells?
For endothelial cell culture under chronic shear use C2025. This cartridge allows extra-cellular matrix proteins to be attached to the fiber. One of the few ways to grow endothelial cells (cells that line the interior of blood vessels) under conditions of medium flowing over them (like they experience in the body). When grown under these conditions they behave in a much more in vivo like manner. They lay down flat and form a monolayer, form tight junctions, and certain genes are turned on in response to this shear stress that are not expressed in static culture. Other cell types such as vascular smooth muscle and brain glial cells can be co-cultivated with these endothelial cells. C4300-2025 will yield about 100µg of total RNA for gene expression analysis.
