What are the advantages of dNTP enzymatic synthesis over chemical synthesis?
The enzymatic synthesis of dNTPs uses highly specific enzymatic systems which eliminate impurities and PCR inhibitors, such as modified nucleotides, PPi and deoxynucleoside tetraphosphates. The presence of contaminants resulting from the chemical manufacturing process, such as traces of dNDPs, pyrophosphates or other ionic species (e.g. acetate) interfere with the PCR reaction and may lead to poor yields or no PCR product at all. Chemically synthesised dNTPs, unless thoroughly purified, often contain deoxynucleoside tetraphosphates, which are powerful PCR inhibitors. Chemical synthesis can also lead to deamination and other nucleotide modifications. This is not the case with enzymatic synthesis.
