What are the advantages of cycle sequencing?
The advantages are as follows: • Protocols are robust and easy to perform • Cycle sequencing requires much less template DNA than single-temperature extension methods • Cycle sequencing is more convenient than traditional single-temperature labeling methods that require a chemical denaturation step for double-stranded templates • High temperatures reduce secondary structure, allowing for more complete extension • High temperatures reduce secondary primer-to-template annealing • The same protocol is used for double- and single-stranded DNA • The protocols work well for direct sequencing of PCR products • Difficult templates, such as bacterial artificial chromosomes (BACs), can be sequenced
