What are the advantages of cryo-SEM over other preparation techniques?
Chemical fixation is avoided; a cryo-SEM sample, rapidly frozen, is as close as possible to its natural state. No use of solvents, which can also remove sample components No dehydration, delicate structures are maintained without shrinkage. Fast freezing means chemical balance is well maintained for microanalysis A soft, volatile or liquid sample is stabilised under the electron beam. Freeze fracture and controlled freeze etching allow optimum exposure of internal structure. Cryo-SEM is fast typical preparation time is less than 10 minutes !
