What are the advantages of an Expression Clone compared to a conventional cDNA clone?
Expression Clones are designed for protein production. The insert comprises the complete coding sequence (ORF) of an mRNA (Full ORF Clones), but it does not contain any part of the endogenous UTRs which may strongly hamper protein expression (e.g. by destabilization of mRNA). In Expression Clones, the insert was cloned into an appropriate expression vector (suitable promoter for different expression systems, Shine-Dalgarno / Kozak sequence for efficient translation, different fusion tags). You may choose from various vectors according to your specific requirements.
