What are the advantages in using the gWiz high expression vectors?
The gWiz vectors contain a proprietary modified promoter followed by Intron A from the human cytomegalovirus (CMV) immediate-early (IE) gene. Although the CMV immediate early gene (IE) promoter/enhancer is the most widely used constitutive promoter for expressing high levels of transgene product in many mammalian cells and tissues, not all CMV IE gene promoter/enhancer-based expression vectors are created equal. Depending on the actual CMV IE gene sequences used and the context of the plasmid backbone upon which the expression cassette is constructed, the expression levels can vary as much as two orders of magnitude. The CMV IE promoter sequences contained in the gWIZ vectors have been systemically analyzed and modified. The modifications include removing the sequences that are redundant and deleterious to the high levels of expression while retaining those sequences that are of high transcriptional potency. After coupling the modified promoter with a high-efficiency synthetic transcri
