What are the acceptable methods of euthanasia?
Mice are narcotized by CO2 inhalation and then euthanized by cervical dislocation. Although CO2 alone can euthanize the animals, it must be ascertained if the animals have died (see the NIH guidelines on the use of CO2 alone), thus cervical dislocation is recommended after the use of CO2. CO2 must be delivered from a tank, not from dry ice. The ARC provides tanks and chambers. Let the gas flow for 1 minute to fill the chamber and leave the chamber closed for 5 minutes. The narcotization of mice is rapid, so do not leave the chamber unattended. Death should be ensured by cervical dislocation. In the different facilities at CWRU, mice can be left on the racks in a designated room to be euthanized by ARC staff. The mice must not be overcrowded, and must have sufficient food and water to last through working hours of the next working day. If unweaned pups are left without their mother, the ARC must be notified immediately so that euthanasia may be performed without delay. CO2 for euthanasi
