What are some recommendations for improving binding efficiencyof proteins and DNA (nucleic acids) using secondary amine CovaLink modules?
Use a freshly made methylimidazole and carbodiimide condensing agents for optimal covalent binding of both proteins and DNA (nucleic acids). The binding efficiency of single stranded oligo (a 5′ phosphorylated end of a single-stranded oligomer binds with a phosphoramidate bond) is about 8 – 10% with a typical 25 base oligo.
