What are some good stopping points in the GEArray protocol?
After probe synthesis, you may store the probe at -20 C overnight. If using cDNA GEArrays, remember to denature the probe again before adding it to the hybridization solution the next day by thawing, incubating at 94 C (or boiling) for 2-5 minutes, and then immediately chilling on ice. After pre-hybridization, you may store the damp membrane at -20 C overnight in its original plastic tube after washing the membrane twice with 2X SSC. Repeat pre-hybridization as described in the User Manual the next day or when ready to continue. After chemiluminescent detection particularly if you need a different exposure or missed your chance to capture an image, you may store the damp membrane in its original plastic tube at 4 C overnight. The next day, briefly rinse the membrane twice 3 ml Buffer G (AP Assay Buffer) and repeat the incubation with CDP-Star and exposures as described in the User Manual.
