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What are some factors which may limit accurate study of soil microorganisms?

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What are some factors which may limit accurate study of soil microorganisms?

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nutrients limit bacterial growth in soil, even the samw type of soil does not have the same nutrient content to support bacterial gowth(carbon has been known to be a limiting factor of microbial growth) edaphic and climatic factors( temperature effect) season of the year moisture content, acidity/alkalinity development of soil profile please elaborate more on this, hope this is helpful

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