What are some applications using the TSP (Transferable Solid Phase)?
Reactions such as the ELISA can be performed on the TSP. The pins are coated by submerging in the analyte solution contained in a 96-well plate. Washing and reaction with succeeding antibody or streptavidin conjugates can be performed by transferring the TSP into a washing tray or second 96-well plate filled with the appropriate solution. The TSP is placed into a substrate solution until color is observed and is then removed to ensure a simultaneous start and stop to the enzymatic reaction. The TSP is available sterile for screening hybridoma cells for the production and secretion of monoclonal antibodies. For radioimmune assays, the TSP can be placed directly on X-ray film and exposed for several hours. Only the tips of the pins should be incubated with the radiolabeled reagent. The TSP can also be used with the OmniTray for performing dot blots and for replicating bacterial clones from a 96-well plate. We offer a Tech Note, Vol. 3 No.
Reactions such as the ELISA can be performed on the TSP. The pins are coated by submerging in the analyte solution contained in a 96-well plate. Washing and reaction with succeeding antibody or streptavidin conjugates can be performed by transferring the TSP into a washing tray or second 96-well plate filled with the appropriate solution. The TSP is placed into a substrate solution until color is observed and is then removed to ensure a simultaneous start and stop to the enzymatic reaction. The TSP is available sterile for screening hybridoma cells for the production and secretion of monoclonal antibodies. For radioimmune assays, the TSP can be placed directly on X-ray film and exposed for several hours. Only the tips of the pins should be incubated with the radiolabeled reagent. The TSP can also be used with the OmniTray for performing dot blots and for replicating bacterial clones from a 96-well plate. We offer a Tech Note No.
