What are good fluorochrome dyes to use in multi-color staining for flow cytometry?
PE and FITC and Alexa Fluor 488 are the commonly used dyes for multiple staining with a 488nm laser. The PE-Cy5 tandem dye is bright, excited at 488nm, and has an emission spectrum clearly distinguishable from PE and FITC, making it a good third dye to use in three color staining with a single laser. Other dyes include APC, APC-Cy7, and PE-Cy7 and the Alexa Fluor 647 and 700 and Pacific Blue. Multiple laser flow cytometers are needed in the simultaneous use of these. APC-Alexa Fluor 750 is equivalent to APC-Cy7. Both tandems are excited with the 633nm laser and emit at ~775nm. APC-Alexa Fluor 750 is as bright or slightly brighter and more stable than APC-Cy7; it requires similar compensation. For most experiments they can be used interchangeably. For detailed information about the molecular weight and the absorption and emission wavelengths and intensity of these fluorochrome dyes, see our BestProtocols Fluorescent Dyes Chart or our Fluorochrome Poster. We are more than happy to help y
For detailed information about the absorption and emission wavelengths and intensity of the fluorochrome dyes we offer, see our BestProtocols Fluorescent Dyes Chart or our Fluorochrome Poster. We are more than happy to help you plan your experiments to match the antibodies with the best fluorochromes. Please contact tech@ebioscience.com should you require furthur information.
