What amplification methods can be used after the MeDIP?
There are many compatible methods for amplifying DNA from low starting amounts of DNA (as in ChIP and Methyl DNA IP) including TLAD, WGA, LM-PCR, etc. Amplification of DNA is a method that takes template DNA and makes many copies of it; PCR is one prime example. However, methods such as PCR are exponential amplification methods, meaning that the number of copies made of the template DNA increases at an exponential rate. The largest shortcoming of exponential amplification approaches is their diminished fidelity when amplifying complex mixtures of DNA. In an exponential amplification, variances or fluctuations in the amplification efficiency of a given amplicon early in the amplification will themselves amplify to considerable and detectable differences at the end of the amplification, because of the exponential kinetics. In linear amplification, however, the linear amplification kinetics minimizes such variances in outcome relative to exponential amplification. In addition: • Whole gen
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