What about the administration route for clodronate liposomes in order to deplete alveolar macrophages (AM)?
Liposomes can be administered intranasally as well as intratracheally. The former administration route has been used several times (see literature in our website e.g. : DeHaan, A., Groen, G., Prop, J., Van Rooijen, N., Wilschut, J. 1996. Mucosal immunoadjuvant activity of liposomes: Role of alveolar macrophages. Immunology, 89; 488-493) The difference may be that all liposomes once administered intratracheally, arrive in the lung (alveoli), whereas liposomes administered intranasally (if administration is not well performed) may be spoiled via the oesophagos.
