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We would like to have a BIBAC vector that has different plant selectable markers. Can we remove NPTII and HYG from BIBAC2 and put in different markers?

April 26, 2017bibac bibac2 different hyg Markers nptii plant remove selectable vector

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We would like to have a BIBAC vector that has different plant selectable markers. Can we remove NPTII and HYG from BIBAC2 and put in different markers?

1 Answer

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You don’t need to “remove” anything. The BIBAC backbone, pCH20, has two unique sites adjacent to the left and right T-DNA border sequences (Hamilton, 1997).