We use a lot of Acetonitrile in our extractions. How can i cut down on it?
The quick answer is no we do not use a lot of acetonitrile in UCT extractions. In 2 analyses is the use of aceto prominent 1 Is THC (Blood and Urine): CSTHC In the blood extraction: Aceto can be replaced with either methanol or acetone (carefully adding them to the sample dropwise). In the urine extraction, the Aceto is used to wash the SPE column in either as a 5% solution with 0.1 M Acetate buffer, or 0.1 M HCl: this again can be replaced with a low level of methanol in the wash. With the SSTHC, the precipitation step is the same, the wash is currently 85% DI water 15% Aceto and 1 % Ammonium Hydroxide, this could be altered to a lower % of methanol (approx, 7-10%) and increase the DI Water content. 2 Is the Benzo analysis which currently uses 20% Aceto in 0.1 M phosphate buffer, this again can be replaced with approx. 10% methanol, or alternatively the column can be washed with DI Water, 0.1 M acid, dried, washed with hexane, Dried, then eluted with Dichloromethane/ IPA/ Ammonium Hyd
