Usually customers grow cells for 3 to 4 days and at certain density, they subculture cells. How can they subculture in this matrix?
Answer. Expand by dissolving matrix using calcium binding solution, Versene or sodium citrate. Remaining spheroids can be dissociated with TrypLE, counted and expanded into other culture vessels or into new AlgiMatrix™ sponges. These steps are illustrated in our instruction manual that is supplied with each kit.
