Tris Buffers – What electrodes can I use?
TRIS buffers are used by biochemists to control pH in the physiological range (about 7 to 8 pH) because phosphates cause undesirable side reactions with the biological substances in their test samples. However, when pH measurements are to be made on these solutions, another type of “undesirable side reaction”, involving the pH electrode system, must be recognized. The common Silver-Silver Chloride reference electrode used with most combination pH electrodes has a Potassium Chloride salt-bridge which is saturated with Silver Chloride. This salt-bridge system works well in most samples, but not in biological samples containing proteins or related materials. The quite low concentration of Silver ion (about 0.0001 M) is sufficient to react with proteins and produce an insoluble precipitate in the porous liquid junction structure of the electrode and thus cause errors in pH measurement due to the development of substantial “liquid junction” potentials across this plug of precipitate. This p
Related Questions
- Researcher cannot use the recommended Tris Buffer due to the cells. They want to know if they can use these enzymes in PBS or RPMI 1640 buffers, and if so, will there be any loss of activity?
- Does PPS Silent Surfactant dissolve in ordinary buffers (e.g. Tris or Ammonium Bicarbonate) and organic solvents?
- Are Sensorexs pH electrodes compatible with Tris Buffers ?
