This protein datasheet indicates I need to use a cross-linking antibody, MAB050, for biological activity. What is this antibody and is it really necessary?
The antibody is directed against a 6x histidine repeat. The proteins that recommend the use of the cross-linker have a 6x his-tag. R&D Systems uses this antibody to cross-link the his-tags causing the protein to have a greater biological activity. This is often used for proteins that require receptor trimerization for signal transduction. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the product insert.
