Theres precious little information in Becker and Purves about protein/polypeptide separation and purification techniques. Can you suggest any other reading in these areas?
Most biochemistry textbooks treat most of the protein purification methods mentioned in lecture. However, the treament is often more detailed than is necessary or desirable for this introductory course. We prepared a rather lengthy handout to address this deficiency of treatment in the recommended texts. I have recently read the section on protein separation techniques in Lodish et al.’s Molecular Cell Biology (3rd Ed., Freeman) and find it close to the level of the lecture. Check the index for electrophoresis to find the subject. 14. I don’t understand the real meaning of the Km value and how it relates to an enzyme or rate of the reaction for that matter. It could be defined as [s} when Vmax = 1/2 Vmax but that doesn’t really help me to understand what it is used for. It can also be defined as a “kinetic paramater” or as compared to [s] when it is much greater than or much less than the Km. I am having some trouble understanding the whole picture. Is there a definition of the actual
