There seems to be a decrease in the reduction of alamarBlue® if I use a long incubation time?
alamarBlue® contains proprietary buffering agents, which maintain the equilibrium between the oxidized and reduced forms of the indicator. If alamarBlue® is used for extended incubation periods, there is a possibility that the buffering agents will reach their maximum buffering efficiency. As this point, the equilibrium between the oxidized and reduced forms of alamarBlue® is disrupted, and a colorless form of the dye may be obtained. This could result in an apparent bleaching of the solution, and a decrease in the amount of the reduced (red) form of alamarBlue®. If a decrease in reduction of the solution is observed at a certain point, or if any apparent bleaching is observed, we recommend that the incubation time is shortened. Please refer to Worked example 1 for details for control experiments to optimize incubation time for alamarBlue®. Alternatively, if the length of the experiment is longer than the optimal alamarBlue® incubation time, we suggest that an endpoint test is used. Th
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- There seems to be a decrease in the reduction of alamarBlue® if I use a long incubation time?
