There are two papers in two journals, by two different teams of people. Whats the difference?
Both teams used samples from the same specimen, Vindija (Vi) 80 — so in principle, they are sequencing the same genome. The difference between the two comes from their methods of sequencing the DNA. The Rubin group (Noonan et al. 2006) is using a metagenomics method based on the creation of a clone library from the ancient DNA. To make a clone library, DNA from a sample is cut with a restriction enzyme, which cuts the DNA at every place that it displays the same short sequence (usually 4- or 6-bp sequences, such as “ATTA”). The short fragments of DNA are mixed together and bound to vectors that can be maintained and replicated in cells. This is the “cloning” process, and the “library” consists of all the short fragments, which (hopefully) overlap each other so that they can be reconstructed. People have made libraries for a long time. For example, the entire mRNA complement in a given tissue type may be made into a library of complementary DNA (cDNA).
Both teams used samples from the same specimen, Vindija (Vi) 80 — so in principle, they are sequencing the same genome. The difference between the two comes from their methods of sequencing the DNA. The Rubin group (Noonan et al. 2006) is using a metagenomics method based on the creation of a clone library from the ancient DNA. To make a clone library, DNA from a sample is cut with a restriction enzyme, which cuts the DNA at every place that it displays the same short sequence (usually 4- or 6-bp sequences, such as “ATTA”). The short fragments of DNA are mixed together and bound to vectors that can be maintained and replicated in cells. This is the “cloning” process, and the “library” consists of all the short fragments, which (hopefully) overlap each other so that they can be reconstructed. People have made libraries for a long time. For example, the entire mRNA complement in a given tissue type may be made into a library of complementary DNA (cDNA). Once the library is made, it can
