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The wax doesn melt completely when I use an EasyStart tube. What should I do?

April 26, 2017completely melt tube wax

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Posted

The wax doesn melt completely when I use an EasyStart tube. What should I do?

1 Answer

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Posted

A. We recommend setting the thermocycler to complete one step at your specified melting temperature (generally, 94 degrees Celcius) for a period of 1-5 minutes before you begin cycling. This ensures that the wax has a chance to melt completely before you begin cycling through cooler steps and your template will denature completely to give you better, more uniform annealing.