The results we get in our lab are not as good as they used to be. Could it be the electroporation equipment?
Possibly. Our technical group can fax to you the appropriate tests to evaluate the electroporation generator you are using. If the instrument is not used for long periods of time, the capacitors in electroporation generators age and may not have the same output. In these situations, re-optimization is suggested and can usually be accomplished by varying the voltage slightly. Most reports of an efficiency decrease may be traced to changing biological parameters or technique. Electroporation is sometimes dependent on temperature, volume, buffer compositions, etc. Thus, it is wise to take a good look at these factors before exploring equipment failures.
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