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The RampUP protocol calls for RNA in a volume of 2µL. If an RNA sample is in a volume of more than 2µL, can it be concentrated?

April 26, 2017Concentrated Protocol rampup RNA sample volume

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The RampUP protocol calls for RNA in a volume of 2µL. If an RNA sample is in a volume of more than 2µL, can it be concentrated?

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Yes, provided the RNA is suspended in water. If so, follow any of the below procedures to concentrate the RNA sample: -SpeedVac or similar vacuum concentrator -Lyophilization -Evaporation in a 60°C heat block -Microcon® YM-3 concentrator (Millipore™ cat. no.