The membrane appears to have more red on the edge.What does that mean?
This is not of concern as long as the areas where the lines form are relatively clear. This phenomenon can be caused by non-uniform sections within the membrane where the flow is faster or slower than other sections. If in doubt, you should allow the strip to continue to run the full 45 minutes and then read the result. Often, the strip will clear up after some time has elapsed to allow all of the gold conjugate to be released from the conjugate pad.