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The isotype of my primary antibody will not fix complement. Can I use a secondary antibody that fixes complement to deplete cells labeled with this primary antibody?

April 26, 2017Antibody cells complement deplete Fix Fixes isotype labeled primary Secondary

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The isotype of my primary antibody will not fix complement. Can I use a secondary antibody that fixes complement to deplete cells labeled with this primary antibody?

1 Answer

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10 Posted

This has been tried in the past but with limited success, therefore we do not recommend it.