The final pellet I get after using Enhance S Plus contains a large percentage of non-motile spermatozoa, is Enhance S Plus not working correctly?
High sperm yield with bad selection is symptomatic for too high centrifugation speeds. Calculate the optimal centrifuge speed using this formula: rpm = Square root {g/(1.118 x r)}. Replace “r” with the radius of the centrifuge in mm. The radius should be measured from the center of the rotor to the most distant point of the centrifuge tube => in case of a swing-bucket rotor, measure with the bucked in its horizontal position. Replace “g” with the desired g-force (e.g. 300g).
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