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The contaminated solution does not flow through the cartridge. Why?

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The contaminated solution does not flow through the cartridge. Why?

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A22: The cartridge is susceptible to blockage by pieces of agarose or other particles. This will eventually completely abolish the buffer flow. The Bind-ETTM is therefore equipped with a sieve in the lid of the reservoir. All solutions must be poured through the sieve to eliminate all particles or gel pieces.

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