The collected antibody is on a higher volume than I started. How much of that should I use to probe a second blot?
Related Questions
- I would like to confirm a positive signal for a kinase by IP-Western blot using the capture antibody on the array. Can I inquire if the antibody is listed in R&D Systems’ catalog?
- The collected antibody is on a higher volume than I started. How much of that should I use to probe a second blot?
- What is the minimum volume of antibody required to completely cover the blot holder surface area?
