The C. elegans ORFeome represents pools of ORFs, what does that mean?
After PCR amplification with gene-specific primers, a pool of wildtype as well as mutated PCR products (of all splice-variants of that ORF present in the cDNA library) are cloned into the Gateway vector. In order to obtain a wild-type sequence of each splice-variant of your gene of interest, single cells have to be picked and their vectors sequenced.
