Systematic inhibition of all molecules within a given molecule interaction pathway (RNAi and other)?
Such strategies are ‘systematic’ only at a small scale which is a major limitation (testing of a few to a few dozens molecules). They also require the previous definition and delimitation of the pathway. Our expertise in human pathway building unambiguously shows that to be coherent, a pathway sustaining even a limited physiological function (ex.: signal transduction from a ligand / receptor couple) systematically includes more than 100 genes / proteins. Only dynamic models provide really systematic analyses which means testing hundreds of molecules. Dynamic modeling also provides the ability to systematically assess associations of effects (co-targeting) which is not feasible through other techniques.
