Summarize the b2-Glycoprotein 1 test kit. What is the normal range?
Patient serum is diluted with sample diluent and incubated in microwells coated with human b2GPI. Antibodies to b2GPI present in the sample will bind to the coated wells. After washing, enzyme conjugated anti-human IgG, IgM, or IgA immunoglobulin is added, the wells are washed again, substrate added, and color development measured in a spectrophotometer at 450 nm. With a simple calculation, semi-quantitative results in G, M, or A units are available in less than 1 hour. The normal range is less than 20 units for each isotype (IgG, IgM, or IgA).
