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Spotting Solution typically provide?

April 26, 2017solution spotting

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10 Posted

Spotting Solution typically provide?

1 Answer

0

10 Posted

1. It depends how much spotting mix will be put into one well. We recommend 50 μM final oligo concentration in the spotting mix. Then the spotting mix consists of: 1 part oligos (200 μM) : 3 part spotting solution (1). (NB! Asper provides 2 concentrate). If you put 50μl spotting mix into one well (37,5 μl 1 Spotting Solution), then 50 ml of 2 spotting solution is enough for 2666 wells.